| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kistler, Shelby N (10)
|
NCYBAC |
35.04 |
34.70 |
-0.34 |
| 2 |
Thorpe, Olivia R (9)
|
SCRAYS |
35.05 |
35.22 |
0.17 |
| 3 |
Ono, Haruka (10)
|
SCRAYS |
|
35.84 |
- |
| 4 |
Corbi, Julia R (10)
|
SCRAYS |
35.01 |
35.92 |
0.91 |
| 5 |
Basara, Grace M (10)
|
SCRAYS |
37.78 |
36.70 |
-1.08 |
| 6 |
Babani, Elsa N (9)
|
NCATOM |
38.80 |
36.81 |
-1.99 |
| 7 |
Meehan, Amelia M (10)
|
SCRAYS |
36.56 |
37.18 |
0.62 |
| 8 |
Ullman, Elise O (10)
|
NCMSA |
39.67 |
37.66 |
-2.01 |
| 9 |
Vidarsson, Louise F (10)
|
NCMSA |
37.67 |
37.74 |
0.07 |
| 10 |
Ndubuisi, Bozena Y (9)
|
SCCS |
38.97 |
37.99 |
-0.98 |
| 11 |
Faulkenberry, Madison B (10)
|
SCRAYS |
56.38 |
38.83 |
-17.55 |
| 12 |
Heinle, Natalie C (10)
|
SCRAYS |
39.44 |
39.35 |
-0.09 |
| 13 |
Druckemiller, Lily A (10)
|
SCRAYS |
37.54 |
39.78 |
2.24 |
| 14 |
Bullins, Logan A (9)
|
NCATOM |
43.04 |
40.76 |
-2.28 |
| 15 |
Eckert, Hannah G (10)
|
SCRAYS |
45.60 |
40.86 |
-4.74 |
| 16 |
Knox, Virginia E (10)
|
NCATOM |
42.04 |
42.58 |
0.54 |
| 17 |
Higbee, Addison S (10)
|
NCATOM |
|
42.91 |
- |
| 18 |
Ivan, Dominique (9)
|
NCMSA |
47.96 |
43.49 |
-4.47 |
| 19 |
Schutzman, Eleanora R (10)
|
SCRAYS |
44.82 |
43.59 |
-1.23 |
| 20 |
Korn, Emma M (9)
|
NCATOM |
43.54 |
43.94 |
0.40 |
| 21 |
Kodakandla, Amukta (10)
|
SCRAYS |
51.80 |
46.44 |
-5.36 |
| 22 |
Yang, Avery J (8)
|
NCATOM |
47.00 |
46.61 |
-0.39 |
| 23 |
Harrison, Hannah N (10)
|
SCRAYS |
47.37 |
46.81 |
-0.56 |
| 24 |
Price, Anna R (10)
|
NCATOM |
47.32 |
47.20 |
-0.12 |
| 25 |
Roelandts, Samantha D (8)
|
SCRAYS |
|
48.24 |
- |
| 26 |
Taylor, Riley J (9)
|
NCATOM |
|
48.89 |
- |
| 27 |
Shaw, Blakely B (8)
|
NCATOM |
|
49.00 |
- |
| 28 |
Wilkerson, Amelia H (10)
|
SCRAYS |
47.96 |
49.53 |
1.57 |
| 29 |
Dawson, Isabella M (8)
|
NCATOM |
53.62 |
50.62 |
-3.00 |
| 30 |
Hightower, Maci C (8)
|
SCRAYS |
|
54.36 |
- |
| 31 |
Gaza, Lauren E (7)
|
SCCS |
|
1:01.65 |
- |
|
Walter, Aspen S (8)
|
SCCA |
|
NS |
- |